Multiplexing SERS nanotags for the imaging of differentiated mouse embryonic stem cells (mESC) and detection of teratoma in vivo

被引：0

作者：

Samanta, Animesh ^{[1
]}

Das, Raj Kumar ^{[2
]}

Park, Sung Jin ^{[1
]}

Maiti, Kaustabh Kumar ^{[3
]}

Chang, Young Tae ^{[1
,2
]}

机构：

[1] Singapore Bioimaging Consortium, Agcy Sci Technol & Res A STAR, Singapore, Singapore

[2] Natl Univ Singapore, Dept Chem, 3 Sci Dr 3, Singapore 117543, Singapore

[3] CSIR Natl Inst Interdisciplinary Sci Technol, Chem Sci Technol Div CSTD, Organ Chem sect, Ind Estate, Thiruvananthapuram 695019, Kerala, India

来源：

AMERICAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING | 2014年 / 4卷 / 02期

关键词：

Gold nanoparticles; multiplexing; teratoma; biocompatible; nanotags; in vivo; SERS; cell mapping; differentiated germ layers; mesoderm; endoderm; ectoderm;

D O I：

暂无

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Herein, we report fifty four membered, a new set of novel NIR Raman reporters and CyRLA-572 has been selected to be the best among them considering the signal intensity and stability. This new reporter molecule is an excellent partner with our in house Raman reporters (Cy7LA and Cy7.5LA). These three NIR Raman reporters are adsorbed on the gold nanoparticles to obtain their corresponding unique SERS fingerprints in which three individual characteristic peaks are capable to multiplex among them. These multiplexed Raman reporters are applied to develop biocompatible and specific targeting SERS nanotags after tagging with specific antibodies. These multiplex targeted SERS nanotags are applied to detect three targeting receptors in differentiated mouse embryonic stem cells (mESCs) consisting three germ layers such as ectoderm, mesoderm and endoderm. After successful recognition of cells by SERS techniques, we detect simultaneously three germ layers in teratoma which is a monster tumor formed from mESC cells in animal xenograft model.

引用

页码：114 / +

页数：23