Gastric fundus submucosa as a site for islets transplantation: An experimental study

被引：1

作者：

Antunes de Mesquita, Gustavo Heluani ^{[1
]}

Jardim, Yuri Justi ^{[1
]}

Iuamoto, Leandro Ryuchi ^{[1
]}

Suguita, Fabio Yuji ^{[1
]}

Essu, Felipe Futema ^{[1
]}

Oliveira, Lucas Torres ^{[1
]}

Meyer, Alberto ^{[2
,3
]}

Crescenzi, Alessandra ^{[3
]}

Rocha-Santos, Vinicius ^{[3
]}

Galvao, Flavio H. F. ^{[3
]}

Andraus, Wellington ^{[2
]}

Chaib, Eleazar ^{[3
]}

Carneiro D'Albuquerque, Luiz Augusto ^{[2
,3
]}

机构：

[1] Univ Sao Paulo, Fac Med FMUSP, Dr Nicolau de Souza Queiros St,406,Apartment 92, Sao Paulo, SP, Brazil

[2] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Dept Gastroenterol, Sao Paulo, SP, Brazil

[3] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Div Transplante Figado,LIM 37, Sao Paulo, SP, Brazil

来源：

ANNALS OF MEDICINE AND SURGERY | 2018年 / 28卷

基金：

巴西圣保罗研究基金会;

关键词：

Gastric fundus submucosa; Islets transplantation; Type; 1; diabetes;

D O I：

10.1016/j.amsu.2017.12.006

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Islets of Langerhans transplantation is a promising alternative for glycemic control in patients with type 1 diabetes. The graft site is a factor that has large impact on the functioning of this transplant, and the stomach appears to be a promising location. Our objective is to describe a new experimental model for the grafting of Islets of Langerhans in rat stomachs. Methodology: Islets of Langerhans were extracted from 45 isogenic male rats of the Lewis lineage and transplanted into 9 isogenic rats of the Wistar lineage; 5 in the gastric body submucosa, and 4 in the gastric fundus submucosa. Normoglycemia was defined as two successive measurements of < 250 mg/dL. No immunosuppression was used. The two groups glycemia control improvement were compared with t-student test. Results: The results obtained following the transplantation of the islets in 9 rats showed between 995 and 2310 islets transplanted (mean of 1367). The rats from the gastric submucosa group had a better glycemic level improvement, with a confidence equal to 83.94%. Conclusion: Islets graft into the gastric fundus submucosa is a viable model with potential for adequate glycemic control. This model gives potential for new perspectives and future studies in this area.

引用

页码：1 / 5

页数：5

共 12 条

[1] Evaluation of alternative sites for islet transplantation in the minipig: Interest and limits of the gastric submucosa [J].