REGULATION OF TREHALOSE METABOLISM IN SACCHAROMYCES-CEREVISIAE MUTANTS DURING TEMPERATURE SHIFTS

被引：33
作者：
PANEK, AC
VANIA, JJM
PASCHOALIN, MF
PANEK, D
机构：
[1] UNIV FED RIO DE JANEIRO,INST QUIM,DEPT BIOQUIM,RIO DE JANEIRO,RJ,BRAZIL
[2] UNIV SAO PAULO,INST QUIM,DEPT BIOQUIM,BR-01498 SAO PAULO,BRAZIL
来源：
BIOCHIMIE | 1990年 / 72卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
cAMP-protein phosphorylation; heat shock; Saccharomyces cerevisiae; thermotolerance; trehalose enzymes;
D O I：
10.1016/0300-9084(90)90176-H
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Temperature shifts from 23°C to 36°C resulted in trehalose accumulation in Saccharomyces independently of genetic lesions in the cAMP-protein kinase cascade. In parallel, trehalose 6-phosphate synthase activity increased about 3-fold in all strains: the increase could be inhibited by cycloheximide, suggesting that protein synthesis was required. Heat shock treatment after the temperature shift led to a drastic increase in trehalose activity, and deactivation of the biosynthetic enzyme with a consequent drop in trehalose. Up to now no definite correlation between acquisition of thermotolerance and trehalose accumulation has been made. © 1990.
引用
收藏
页码：77 / 79
页数：3
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