CLONING OF HIGH-AFFINITY METHIONINE TRANSPORT GENES FROM SALMONELLA-TYPHIMURIUM

被引：3
作者：
SHAW, NA
AYLING, PD
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1991年 / 78卷 / 2-3期
关键词：
METHIONINE TRANSPORT; SALMONELLA-TYPHIMURIUM;
D O I：
10.1016/0378-1097(91)90145-Z
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
At least one of the genes encoding a methionine transport system in Salmonella typhimurium has been cloned on a 15 kbp fragment in the lambda-1059 vector, and the region responsible for the transport activity was subcloned in a set of plasmids with inserts ranging from 1.4 to 4.2 kbp in the multicopy plasmid pUC8. Two proteins of M(r) 34 and 40 kDa were expressed from the larger inserts.
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页码：127 / 132
页数：6
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