GENE-EXPRESSION OF FIBROBLAST GROWTH-FACTORS IN HUMAN GLIOMAS AND MENINGIOMAS - DEMONSTRATION OF CELLULAR SOURCE OF BASIC FIBROBLAST GROWTH-FACTOR MESSENGER-RNA AND PEPTIDE IN TUMOR-TISSUES

被引：275
作者：
TAKAHASHI, JA
MORI, H
FUKUMOTO, M
IGARASHI, K
JAYE, M
ODA, Y
KIKUCHI, H
HATANAKA, M
机构：
[1] KYOTO UNIV,INST VIRUS RES,DEPT MOLEC VIROL,HUMAN CANC LAB,KYOTO 606,JAPAN
[2] KYOTO UNIV,FAC MED,DEPT NEUROSURG,KYOTO 606,JAPAN
[3] KYOTO UNIV,FAC MED,DEPT PATHOL,KYOTO 606,JAPAN
[4] TAKEDA CHEM IND LTD,DIV CENT RES,BIOTECHNOL LABS,OSAKA 532,JAPAN
[5] RORER BIOTECHNOL,KING OF PRUSSIA,PA 19406
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 15期
关键词：
Acidic fibroblast growth factor; Brain neoplasm; In situ hybridization; Northern blot analysis; Transforming growth factor type β1;
D O I：
10.1073/pnas.87.15.5710
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meningiomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type β1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.
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收藏
页码：5710 / 5714
页数：5
相关论文
共 26 条
[11]   COLOR MODIFICATION OF DIAMINOBENZIDINE (DAB) PRECIPITATION BY METALLIC-IONS AND ITS APPLICATION FOR DOUBLE IMMUNOHISTOCHEMISTRY [J].
HSU, SM ;
SOBAN, E .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (10) :1079-1082
[12]   EXPRESSION OF ACIDIC FIBROBLAST GROWTH-FACTOR CDNA CONFERS GROWTH ADVANTAGE AND TUMORIGENESIS TO SWISS 3T3-CELLS [J].
JAYE, M ;
LYALL, RM ;
MUDD, R ;
SCHLESSINGER, J ;
SARVER, N .
EMBO JOURNAL, 1988, 7 (04) :963-969
[13]   CLONING AND EXPRESSION OF CDNA-ENCODING HUMAN BASIC FIBROBLAST GROWTH-FACTOR [J].
KUROKAWA, T ;
SASADA, R ;
IWANE, M ;
IGARASHI, K .
FEBS LETTERS, 1987, 213 (01) :189-194
[14]   AN ANGIOGENIC GROWTH-FACTOR IS EXPRESSED IN HUMAN GLIOMA-CELLS [J].
LIBERMANN, TA ;
FRIESEL, R ;
JAYE, M ;
LYALL, RM ;
WESTERMARK, B ;
DROHAN, W ;
SCHMIDT, A ;
MACIAG, T ;
SCHLESSINGER, J .
EMBO JOURNAL, 1987, 6 (06) :1627-1632
[15]   INCREASED EXPRESSION OF GENES FOR BASIC FIBROBLAST GROWTH-FACTOR AND TRANSFORMING GROWTH-FACTOR TYPE-BETA-2 IN HUMAN BENIGN PROSTATIC HYPERPLASIA [J].
MORI, H ;
MAKI, M ;
OISHI, K ;
JAYE, M ;
IGARASHI, K ;
YOSHIDA, O ;
HATANAKA, M .
PROSTATE, 1990, 16 (01) :71-80
[16]   ELEVATED EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR MESSENGER RIBONUCLEIC-ACID IN ACOUSTIC NEUROMAS [J].
MURPHY, PR ;
MYAL, Y ;
SATO, Y ;
SATO, R ;
WEST, M ;
FRIESEN, HG .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (02) :225-231
[17]   EXPRESSION OF HUMAN BASIC FIBROBLAST GROWTH-FACTOR CDNA IN BABY HAMSTER-KIDNEY DERIVED CELLS RESULTS IN AUTONOMOUS CELL-GROWTH [J].
NEUFELD, G ;
MITCHELL, R ;
PONTE, P ;
GOSPODAROWICZ, D .
JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1385-1394
[18]  
RUBINSTEIN LJ, 1972, TUMORS CENTRAL NERVO, P64
[19]   FIBROBLAST GROWTH-FACTOR RELEASE BY BOVINE ENDOTHELIAL-CELLS AND HUMAN ASTROCYTOMA-CELLS IN CULTURE IS DENSITY DEPENDENT [J].
SATO, Y ;
MURPHY, PR ;
SATO, R ;
FRIESEN, HG .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (04) :744-748
[20]   BASIC FIBROBLAST GROWTH-FACTOR IN HUMAN RHABDOMYOSARCOMA CELLS - IMPLICATIONS FOR THE PROLIFERATION AND NEOVASCULARIZATION OF MYOBLAST-DERIVED TUMORS [J].
SCHWEIGERER, L ;
NEUFELD, G ;
MERGIA, A ;
ABRAHAM, JA ;
FIDDES, JC ;
GOSPODAROWICZ, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (03) :842-846
123