GENE-EXPRESSION OF FIBROBLAST GROWTH-FACTORS IN HUMAN GLIOMAS AND MENINGIOMAS - DEMONSTRATION OF CELLULAR SOURCE OF BASIC FIBROBLAST GROWTH-FACTOR MESSENGER-RNA AND PEPTIDE IN TUMOR-TISSUES

被引：275
作者：
TAKAHASHI, JA
MORI, H
FUKUMOTO, M
IGARASHI, K
JAYE, M
ODA, Y
KIKUCHI, H
HATANAKA, M
机构：
[1] KYOTO UNIV,INST VIRUS RES,DEPT MOLEC VIROL,HUMAN CANC LAB,KYOTO 606,JAPAN
[2] KYOTO UNIV,FAC MED,DEPT NEUROSURG,KYOTO 606,JAPAN
[3] KYOTO UNIV,FAC MED,DEPT PATHOL,KYOTO 606,JAPAN
[4] TAKEDA CHEM IND LTD,DIV CENT RES,BIOTECHNOL LABS,OSAKA 532,JAPAN
[5] RORER BIOTECHNOL,KING OF PRUSSIA,PA 19406
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 15期
关键词：
Acidic fibroblast growth factor; Brain neoplasm; In situ hybridization; Northern blot analysis; Transforming growth factor type β1;
D O I：
10.1073/pnas.87.15.5710
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meningiomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type β1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.
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收藏
页码：5710 / 5714
页数：5
相关论文
共 26 条
[1]   NUCLEOTIDE-SEQUENCE OF A BOVINE CLONE ENCODING THE ANGIOGENIC PROTEIN, BASIC FIBROBLAST GROWTH-FACTOR [J].
ABRAHAM, JA ;
MERGIA, A ;
WHANG, JL ;
TUMOLO, A ;
FRIEDMAN, J ;
HJERRILD, KA ;
GOSPODAROWICZ, D ;
FIDDES, JC .
SCIENCE, 1986, 233 (4763) :545-548
[2]   HUMAN BASIC FIBROBLAST GROWTH-FACTOR - NUCLEOTIDE-SEQUENCE AND GENOMIC ORGANIZATION [J].
ABRAHAM, JA ;
WHANG, JL ;
TUMOLO, A ;
MERGIA, A ;
FRIEDMAN, J ;
GOSPODAROWICZ, D ;
FIDDES, JC .
EMBO JOURNAL, 1986, 5 (10) :2523-2528
[3]   MULTIPLE POLYADENYLATION SITES DOWNSTREAM FROM THE HUMAN AFGF GENE ENCODING ACIDIC FIBROBLAST GROWTH-FACTOR [J].
CRUMLEY, GR ;
HOWK, R ;
RAVERA, MW ;
JAYE, M .
GENE, 1989, 85 (02) :489-497
[4]   HUMAN TRANSFORMING GROWTH FACTOR-BETA COMPLEMENTARY-DNA SEQUENCE AND EXPRESSION IN NORMAL AND TRANSFORMED-CELLS [J].
DERYNCK, R ;
JARRETT, JA ;
CHEN, EY ;
EATON, DH ;
BELL, JR ;
ASSOIAN, RK ;
ROBERTS, AB ;
SPORN, MB ;
GOEDDEL, DV .
NATURE, 1985, 316 (6030) :701-705
[5]   BOVINE BRAIN ASTROCYTES EXPRESS BASIC FIBROBLAST GROWTH-FACTOR, A NEUROTROPIC AND ANGIOGENIC MITOGEN [J].
FERRARA, N ;
OUSLEY, F ;
GOSPODAROWICZ, D .
BRAIN RESEARCH, 1988, 462 (02) :223-232
[6]   ANGIOGENIC FACTORS [J].
FOLKMAN, J ;
KLAGSBRUN, M .
SCIENCE, 1987, 235 (4787) :442-447
[7]  
FUKUMOTO M, 1990, IN PRESS MOL BIOL CA
[8]   STRUCTURAL CHARACTERIZATION AND BIOLOGICAL FUNCTIONS OF FIBROBLAST GROWTH-FACTOR [J].
GOSPODAROWICZ, D ;
FERRARA, N ;
SCHWEIGERER, L ;
NEUFELD, G .
ENDOCRINE REVIEWS, 1987, 8 (02) :95-114
[9]   STIMULATION OF DIVISION OF Y1 ADRENAL-CELLS BY A GROWTH-FACTOR ISOLATED FROM BOVINE PITUITARY-GLANDS [J].
GOSPODAROWICZ, D ;
HANDLEY, HH .
ENDOCRINOLOGY, 1975, 97 (01) :102-107
[10]  
GOSPODAROWICZ D, 1987, J CELL PHYSL S, V5, P15
123