CULTURE AND DIFFERENTIATION OF CHONDROCYTES ENTRAPPED IN ALGINATE GELS

被引：73

作者：

GRANDOLFO, M

DANDREA, P

PAOLETTI, S

MARTINA, M

SILVESTRINI, G

BONUCCI, E

VITTUR, F

机构：

[1] UNIV TRIESTE,DIPARTIMENTO BIOCHIM BIOFIS & CHIM MACROMOLEC,VIA A VALERIO 38,I-34127 TRIESTE,ITALY

[2] UNIV ROME LA SAPIENZA,DIPARTIMENTO BIOPATOL UMANA,I-00161 ROME,ITALY

来源：

CALCIFIED TISSUE INTERNATIONAL | 1993年 / 52卷 / 01期

关键词：

CHONDROCYTES; DIFFERENTIATION; SUSPENSION CULTURES; ALGINATE; FURA-2;

D O I：

10.1007/BF00675625

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We studied the response to culture conditions and the differentiative ability in suspension culture in alginate gels of resting chondrocytes from the preosseous cartilage of adult pig scapula. It was found that the maximum rate of chondrocyte duplication is reached at the fourth day in culture whereas the rate of proteoglycan synthesis and alkaline phosphatase expression do not gain a maximum value before the seventh day. During the culture time, the chondrocytes undergo differentiation as it is demonstrated by the alkaline phosphatase specific activity increase and by morphological criteria (hypertrophy, increase of the number of mitochondria per cell, increased endoplasmic reticulum, matrix vesicle production). The alginate gels can be easily dissolved to obtain cell populations in which the variation of cytosolic calcium concentration following a proliferative stimulus can be conveniently observed using the conventional procedure of Fura 2.

引用

页码：42 / 48

页数：7

共 29 条

[21]

SCHMID TM, 1982, J BIOL CHEM, V257, P2444

[22] ENZYMATIC PROPERTIES OF THE CA-2+-BINDING GLYCOPROTEIN ISOLATED FROM PRE-OSSEOUS CARTILAGE [J].

STAGNI, N ;

FURLAN, G ;

VITTUR, F ;

ZANETTI, M ;

BERNARD, BD .

CALCIFIED TISSUE INTERNATIONAL, 1979, 29 (01) :27-32

[23] CALCIFICATION OF INVITRO DEVELOPED HYPERTROPHIC CARTILAGE [J].

TACCHETTI, C ;

QUARTO, R ;

CAMPANILE, G ;

CANCEDDA, R .

DEVELOPMENTAL BIOLOGY, 1989, 132 (02) :442-447

[24]

Takagi M., 1990, ULTRASTRUCTURE SKELE, P111

[25] CHEMICAL MODIFICATIONS OF CARTILAGE MATRIX DURING ENDOCRONDRAL CALCIFICATION [J].

VITTUR, F ;

PUGLIARE.MC ;

BERNARD, BD .

EXPERIENTIA, 1971, 27 (02) :126-&

[26]

VITTUR F, 1977, B MOL BIOL MED, V2, P189

[27]

von der Mark H, 1976, Dev Biol, V48, P237, DOI 10.1016/0012-1606(76)90088-9

[28]

WU LNY, 1989, J BIOL CHEM, V264, P21346

[29]

WUTHIER RE, 1985, J BIOL CHEM, V260, P5972

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