EXPRESSION OF C-MET PROTOONCOGENE IN COS CELLS INDUCES THE SIGNAL TRANSDUCING HIGH-AFFINITY RECEPTOR FOR HEPATOCYTE GROWTH-FACTOR

被引：81

作者：

HIGUCHI, O

MIZUNO, K

VANDEWOUDE, GF

NAKAMURA, T

机构：

[1] KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

[2] NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21701

来源：

FEBS LETTERS | 1992年 / 301卷 / 03期

关键词：

HEPATOCYTE GROWTH FACTOR; HGF; HGF RECEPTOR; C-MET PROTOONCOGENE; MITOGENIC SIGNAL;

D O I：

10.1016/0014-5793(92)80257-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By transfection of the expression plasmid containing a human c-met cDNA into COS-7 cells, high-affinity binding sites specific for HGF with a K(d) value of 30 pM were newly detected. Furthermore, only in the c-met transfected COS-7 cells, but not in the control COS-7 cells, DNA synthesis was markedly induced in response to HGF. Thus, transient expression of exogenous c-met cDNA resulted in the appearance of high-affinity receptor for HGF and conversion of the normally non-responsive COS-7 cells into the HGF-responsive cells. These results provide evidence for identifying the c-met product as a signal transducing high-affinity receptor for HGF.

引用

收藏

页码：282 / 286

页数：5

相关论文

共 26 条

[1] IDENTIFICATION OF THE HEPATOCYTE GROWTH-FACTOR RECEPTOR AS THE C-MET PROTOONCOGENE PRODUCT [J].

BOTTARO, DP ;

RUBIN, JS ;

FALETTO, DL ;

CHAN, AML ;

KMIECIK, TE ;

VANDEWOUDE, GF ;

AARONSON, SA .

SCIENCE, 1991, 251 (4995) :802-804

[2]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[3]

FURLONG RA, 1991, J CELL SCI, V100, P173

[4] CHARACTERIZATION OF THE TPR-MET ONCOGENE-P65 AND THE MET PROTOONCOGENE-P140 PROTEIN-TYROSINE KINASES [J].

GONZATTIHACES, M ;

SETH, A ;

PARK, M ;

COPELAND, T ;

OROSZLAN, S ;

VANDEWOUDE, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :21-25

[5] HIGH-AFFINITY NGF BINDING REQUIRES COEXPRESSION OF THE TRK PROTOONCOGENE AND THE LOW-AFFINITY NGF RECEPTOR [J].

HEMPSTEAD, BL ;

MARTINZANCA, D ;

KAPLAN, DR ;

PARADA, LF ;

CHAO, MV .

NATURE, 1991, 350 (6320) :678-683

[6] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[7] IDENTIFICATION AND CHANGE IN THE RECEPTOR FOR HEPATOCYTE GROWTH-FACTOR IN RAT-LIVER AFTER PARTIAL-HEPATECTOMY OR INDUCED HEPATITIS [J].

HIGUCHI, O ;

NAKAMURA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (02) :599-607

[8] HEPATOCYTE GROWTH-FACTOR IS A POTENT MITOGEN FOR CULTURED RABBIT RENAL TUBULAR EPITHELIAL-CELLS [J].

IGAWA, T ;

KANDA, S ;

KANETAKE, H ;

SAITOH, Y ;

ICHIHARA, A ;

TOMITA, Y ;

NAKAMURA, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (02) :831-838

[9]

KAUFMAN RJ, 1990, METHOD ENZYMOL, V185, P487, DOI 10.1016/0076-6879(90)85041-L

[10] DETECTION OF MESSENGER-RNA FOR THE TAX1 REX1 GENE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I IN FRESH PERIPHERAL-BLOOD MONONUCLEAR-CELLS OF ADULT T-CELL LEUKEMIA PATIENTS AND VIRAL CARRIERS BY USING THE POLYMERASE CHAIN-REACTION [J].

KINOSHITA, T ;

SHIMOYAMA, M ;

TOBINAI, K ;

ITO, M ;

ITO, S ;

IKEDA, S ;

TAJIMA, K ;

SHIMOTOHNO, K ;

SUGIMURA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5620-5624