Skeletal muscle fiber-type-specific changes in markers of capillary and mitochondrial content after low-volume interval training in overweight women

被引：39
作者：
Tan, Rachel [1 ]
Nederveen, Joshua P. [1 ]
Gillen, Jenna B. [1 ]
Joanisse, Sophie [1 ]
Parise, Gianni [1 ]
Tarnopolsky, Mark A. [2 ]
Gibala, Martin J. [1 ]
机构：
[1] McMaster Univ, Dept Kinesiol, Hamilton, ON, Canada
[2] McMaster Univ, Pediat & Med, Hamilton, ON, Canada
来源：
PHYSIOLOGICAL REPORTS | 2018年 / 6卷 / 05期
基金：
加拿大自然科学与工程研究理事会;
关键词：
angiogenesis; capillarization; COXIV; high-intensity interval exercise; oxidative capacity;
D O I：
10.14814/phy2.13597
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
High-intensity interval training (HIIT) enhances skeletal muscle oxygen delivery and utilization but data are limited regarding fiber-specific adaptations in humans. We examined the effect of 18 sessions of HIIT (10x60-sec cycling intervals at similar to 90% HRmax, interspersed by 60-sec of recovery) over 6weeks on markers of microvascular density and oxidative capacity in type I and II fibers in healthy but sedentary young women (Age: 26 +/- 7years; BMI: 30 +/- 4kg.m(-2); VO2peak: 2.16 +/- 0.45L.m(-1)). Immunohistochemical analyses of muscle cross sections revealed a training-induced increase in capillary contacts per fiber in type I fibers (PRE: 4.38 +/- 0.37 vs. POST: 5.17 +/- 0.80; main effect, P<0.05) and type II fibers (PRE: 4.24 +/- 0.55 vs. POST: 4.92 +/- 0.54; main effect, P<0.05). The capillary-to-fiber ratio also increased after training in type I fibers (PRE: 1.53 +/- 1.44 vs. POST: 1.88 +/- 0.38; main effect, P<0.05) and type II fibers (PRE: 1.45 +/- 0.19 vs. POST: 1.76 +/- 0.27; main effect, P<0.05). Muscle oxidative capacity as reflected by the protein content of cytochrome oxidase IV also increased after training in type I fibers (PRE: 3500 +/- 858 vs. POST: 4442 +/- 1377 arbitrary units; main effect, P<0.01) and type II fibers (PRE: 2632 +/- 629 vs. POST: 3863 +/- 1307 arbitrary units; main effect, P<0.01). We conclude that short-term HIIT in previously inactive women similarly increases markers of capillary density and mitochondrial content in type I and type II fibers.
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