POLYETHENOADENOSINE PHOSPHATE AS A FLUOROGENIC SUBSTRATE FOR BARNASE

被引：10

作者：

FITZGERALD, PC ^{[1
]}

HARTLEY, RW ^{[1
]}

机构：

[1] NIDDKD,CELLULAR & DEV BIOL LAB,BETHESDA,MD 20892

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 214卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyethenoadenosine phosphate is rapidly hydrolyzed by barnase with an accompanying large increase in fluorescence. At pH 8, 0.2 M ammonium acetate, the initial rate of fluorescence increase is proportional to barnase concentration and thus provides an excellent quantitative assay for its activity. The preparation and use of this substrate in the barnase assay is presented in detail. © 1993 Academic Press, Inc.

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页码：544 / 547

页数：4

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