RESTRICTION ENDONUCLEASES DO INDUCE SISTER-CHROMATID EXCHANGES IN CHINESE-HAMSTER OVARY CELLS

被引：9

作者：

BALAJEE, AS ^{[1
]}

NATARAJAN, AT ^{[1
]}

机构：

[1] LEIDEN UNIV,MGC,DEPT RADIAT GENET & CHEM MUTAGENESIS,SYLVIUS LABS,2333 AL LEIDEN,NETHERLANDS

来源：

MUTATION RESEARCH | 1993年 / 302卷 / 01期

关键词：

SISTER-CHROMATID EXCHANGE; RESTRICTION ENDONUCLEASE; DOUBLE-STRAND BREAKS; STREPTOLYSIN-O;

D O I：

10.1016/0165-7992(93)90086-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Restriction endonucleases (REs) AluI, EcoRI, MspI, PvuII and SmaI were introduced into exponentially growing Chinese hamster ovary (CHO) cells permeabilised by the bacterial cytotoxin, streptolysin O (SLO). Sister-chromatid exchanges (SCEs) were scored in posttreatment metaphases following recovery times of 20 and 24 h. At both fixation times, all five REs tested were found to increase the frequencies of SCEs compared with that of SLO-treated controls. Among the REs used, AluI and PvuII were found to be most effective, inducing on average a 2-3-fold increase, with a proportion of cells exhibiting more than 80 SCEs. These results confirm the earlier findings of Natarajan et al. (1985) and tend to suggest that double-strand breaks (DSBs) induced by REs are involved in the formation of SCEs.

引用

页码：25 / 31

页数：7

共 21 条

[1] INDUCTION OF SISTER CHROMATID EXCHANGES (SCE) IN G0 LYMPHOCYTES BY PU-238 ALPHA-PARTICLES [J].

AGHAMOHAMMADI, SZ ;

GOODHEAD, DT ;

SAVAGE, JRK .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1988, 53 (06) :909-915

[2] LONG REGIONS OF SINGLE-STRANDED-DNA IN HUMAN-CELLS [J].

BJURSELL, G ;

GUSSANDER, E ;

LINDAHL, T .

NATURE, 1979, 280 (5721) :420-423

[3] INDUCTION OF CHROMOSOMAL DAMAGE BY RESTRICTION ENDONUCLEASE IN CHO CELLS PORATED WITH STREPTOLYSIN-O [J].

BRYANT, PE .

MUTATION RESEARCH, 1992, 268 (01) :27-34

[4] ENZYMATIC RESTRICTION OF MAMMALIAN-CELL DNA USING PVU-II AND BAM-H1 - EVIDENCE FOR THE DOUBLE-STRAND BREAK ORIGIN OF CHROMOSOMAL-ABERRATIONS [J].

BRYANT, PE .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1984, 46 (01) :57-65

[5] DIFFERENCES IN ACCUMULATION OF BLUNT-ENDED AND COHESIVE-ENDED DOUBLE-STRAND BREAKS GENERATED BY RESTRICTION ENDONUCLEASES IN ELECTROPORATED CHO CELLS [J].

COSTA, ND ;

BRYANT, PE .

MUTATION RESEARCH, 1991, 254 (03) :239-246

[6] THE RESTRICTION ENDONUCLEASE ALUI INDUCES SISTER CHROMATID EXCHANGE IN CHINESE-HAMSTER OVARY CELLS [J].

FOLLE, GA ;

JOHANNES, C ;

MECHOSO, BH ;

MEDINA, II ;

OBE, G .

MUTAGENESIS, 1992, 7 (04) :291-294

[7] EFFECTS OF INHIBITORS OF DNA-SYNTHESIS ON SPONTANEOUS AND ULTRAVIOLET LIGHT-INDUCED SISTER-CHROMATID EXCHANGES IN CHINESE-HAMSTER CELLS [J].

ISHII, Y ;

BENDER, MA .

MUTATION RESEARCH, 1980, 79 (01) :19-32

[8] MECHANISMS FOR SISTER CHROMATID EXCHANGES AND THEIR RELATION TO PRODUCTION OF CHROMOSOMAL-ABERRATIONS [J].

KATO, H .

CHROMOSOMA, 1977, 59 (03) :179-191

[9]

MCCLELLAND M, 1987, NUCLEIC ACIDS RES, V15, P219

[10] CLEAVAGE OF DNA - RNA HYBRIDS BY TYPE-II RESTRICTION ENZYMES [J].

MOLLOY, PL ;

SYMONS, RH .

NUCLEIC ACIDS RESEARCH, 1980, 8 (13) :2939-2946

← 1 2 3 →