PLATELET COLLECTION BY APHERESIS WITH REDUCED USE OF CITRATE - QUANTITATIVE AND QUALITATIVE EVALUATION OF 3 PROTOCOLS

被引：1

作者：

MOHKLAREN, J ^{[1
]}

BERRIOT, N ^{[1
]}

LECRUBIER, C ^{[1
]}

SAMAMA, M ^{[1
]}

ANDREU, G ^{[1
]}

机构：

[1] HOTEL DIEU PARIS,CENT HEMATOL LAB,F-75181 PARIS 04,FRANCE

来源：

TRANSFUSION CLINIQUE ET BIOLOGIQUE | 1995年 / 2卷 / 03期

关键词：

APHERESIS; BLOOD DONATION; PLATELET AGGREGATION;

D O I：

10.1016/S1246-7820(05)80045-X

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Three protocols of platelet collection by apheresis on Cobe Spectra with different citrate concentrations were compared. First we determined until what level we could diminish the ratio anticoagulant/whole blood flow to enhance the efficiency without clotting in the cell separator by platelet aggregates. We found that protocol 2 had a better platelet collection efficiency than the standard protocol 1 : respectively 59 % versus 52 %. Protocol 3 had even a better efficiency but, due to the risk of clotting (5 % of the procedures) we rejected it for routine use. We compared in vitro platelet fonctions of protocols I and 2 by aggregometry in vitro. The results with the different aggregating agents indicated that the less citrated environment kept the platelets more fonctional in vitro. A disadvantage of protocol 2 compared to the standard protocol is the more important number of platelet concentrates that contain more than 10(6) leucocytes.

引用

页码：181 / 188

页数：8