TIME-DEPENDENT AND VOLTAGE-DEPENDENT BLOCK OF DELAYED RECTIFIER POTASSIUM CHANNELS BY DOCOSAHEXAENOIC ACID

Docosahexaenoic acid (22:6n3) acts at an extracellular site to produce a voltage- and time-dependent block of the delayed rectifier current (I-K) similar to that classically described for intracellularly applied quaternary ammonia compounds. In dissociated cells from the pineal gland, some long-chain polyunsaturated fatty acids reduced both late sustained (I-K) (for 22:6n3, IC50 = 2.5 +/- 0.3 mu M) and early transient (I-A) (IC50 = 2.0 +/- 0.1 mu M) components of potassium current when applied extracellularly, whereas the monounsaturate oleic acid had minimal efficacy, From comparisons of other related fatty acids, it was determined that there is a structural requirement for polyunsaturation to block I-K. In contrast, chain-elongated 22-carbon polyunsaturates acted similarly to their precursor 20-carbon fatty acids (arachidonic acid and eicosapentanoic acid), Block of I-K by 22:6n3 was accompanied by a dose-dependent acceleration of the current decay in both whole-cell and outside-out membrane patches, and 22:6n3 increased the macroscopic inactivation rate of I-A. The combined ''eicosanoid'' inhibitor eicosatetraenoic acid, when included in the patch pipette, did not antagonize the action of 22:6n3. Instead, eicosatetraenoic acid produced a direct block of I-K when applied extracellularly at high concentrations (25 mu M). Analyses of voltage- and time-dependent block by 22:6n3 support the hypothesis that certain fatty acids directly interact with and preferentially block the open state of some potassium channels. We also describe an interaction between fatty acid block and zinc; 22:6n3 failed to block either I-A or I-K in the presence of zinc or cadmium, whereas extracellular calcium did not affect the response. These studies suggest a possible biological function for 22:6n3 in the nervous system, which may underlie its essential role during neural development.