EFFECT OF SPERM-ANTIBODIES ON ACROSOME REACTION OF HUMAN SPERM USED FOR THE HAMSTER EGG PENETRATION ASSAY

被引：17
|
作者：
FRANCAVILLA, F
ROMANO, R
SANTUCCI, R
机构：
[1] Department of Internal Medicine, University of L'Aquila, Medical School, L'Aquila
来源：
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1991年 / 25卷 / 02期
关键词：
SPERMATOZOA; SPERM ANTIBODIES; ACROSOME REACTION; FERTILIZATION;
D O I：
10.1111/j.1600-0897.1991.tb01067.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The effect of anti-sperm antibodies (ASA) on the rate of acrosome reactions (AR) during "in vitro" capacitation of human sperm used for the hamster egg penetration assay (HEPA) was assessed. Motile sperm suspensions from donors were exposed to several sera and seminal plasma with sperm head-directed ASA, then they were washed and capacitated "in vitro." After capacitation, the proportion of acrosome-reacted viable sperm was assessed by staining with Fluoresceinated Pisum Sativum Agglutinin and supravital stain Hoechst 33258. ASA of any immunoglobulin class did not significantly affect either the AR rate, or the hamster egg penetration rate. In conclusion, interference of ASA on spontaneous AR rate during "in vitro" capacitation can not be advocated as an explanation of the impairment of the interaction of human sperm with egg or its vestments, which have been reported in several studies.
引用
收藏
页码：77 / 80
页数：4
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