A HOMOLOG OF ESCHERICHIA-COLI RECA PROTEIN IN PLASTIDS OF HIGHER-PLANTS

被引：113

作者：

CERUTTI, H ^{[1
]}

OSMAN, M ^{[1
]}

GRANDONI, P ^{[1
]}

JAGENDORF, AT ^{[1
]}

机构：

[1] CORNELL UNIV,PLANT BIOL SECT,ITHACA,NY 14853

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 17期

关键词：

CHLOROPLAST; RECOMBINATION; RECA GENE; DNA DAMAGE; DNA REPAIR;

D O I：

10.1073/pnas.89.17.8068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Studies of chloroplast DNA variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. However, no studies have been done of the biochemical pathways involved. Using a part of a cyanobacterial recA gene as a probe in Southern blots, we have found homologous sequences in total DNA from Pisum sativum and Arabidopsis thaliana and in a cDNA library from Arabidopsis. A cDNA was cloned and sequenced, and its predicted amino acid sequence is 60.7% identical to that of the cyanobacterial RecA protein. This finding is consistent with our other results showing both DNA strand transfer activity and the existence of a protein of the predicted molecular mass crossreactive with antibodies to Escherichia coli RecA in the stroma of pea chloroplasts.

引用

页码：8068 / 8072

页数：5

共 48 条

[1] GENETIC-DAMAGE, MUTATION, AND THE EVOLUTION OF SEX [J].