UREAPLASMA UREALYTICUM UREASE GENES - USE OF A UGA TRYPTOPHAN CODON

被引：95

作者：

BLANCHARD, A

机构：

[1] Laboratoire des Mycoplasmes, Institut Pasteur, Paris, 75724

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 04期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00636.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotide sequence analysis of a Ureaplasma urealyticum DNA fragment, homologous to cloned urease genes of other prokaryotes, revealed three consecutive open reading frames. The molecular weights of the three deduced polypeptides are 11.2kD, 13.6kD and 66.6 kD. These values are consistent with the size of the three subunits previously reported for purified native urease. A significant sequence homology was found between the three polypeptides of the ureaplasmal urease and the single polypeptide of jack bean (Canavalia ensiformis) urease. Codon usage indicates that UGA is a tryptophan codon in this mollicute. Use of polymerase chain reactions has disclosed the existence of genetic polymorphism among the urease genes of different serotypes of U. urealyticum. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：669 / 676

页数：8

共 42 条

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