Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis

被引：17

作者：

Escher, Ulrike ^{[1
,2
]}

Giladi, Eliezer ^{[3
]}

Dunay, Ildiko R. ^{[4
]}

Bereswill, Stefan ^{[1
,2
]}

Gozes, Illana ^{[3
]}

Heimesaat, Markus M. ^{[1
,2
]}

机构：

[1] Humboldt Univ, Freie Univ Berlin, Charite Univ Med Berlin, Berlin, Germany

[2] Berlin Inst Hlth, Inst Microbiol & Infect Immunol, Berlin, Germany

[3] Tel Aviv Univ, Sackler Sch Med, Dept Human Mol Genet & Biochem, Tel Aviv, Israel

[4] Otto von Guericke Univ, Med Fac, Inst Inflammat & Neurodegenerat, Magdeburg, Germany

来源：

EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 8卷 / 02期

关键词：

octapeptide NAP; activity-dependent neuroprotective protein (ADNP); host-pathogen interactions; Toxoplasma gondii; subacute ileitis; host immunity; human gut microbiota; fecal microbiota transplantation; secondary abiotic (gnotobiotic) mice; intestinal; extra-intestinal and systemic immune responses;

D O I：

10.1556/1886.2018.00006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The octapeptide NAP is well known for its neuroprotective properties. We here investigated whether NAP treatment could alleviate pro-inflammatory immune responses during experimental subacute ileitis. To address this, mice with a human gut microbiota were perorally infected with one cyst of Toxoplasma gondii (day 0) and subjected to intraperitoneal synthetic NAP treatment from day 1 until day 8 postinfection (p.i.). Whereas placebo (PLC) control animals displayed subacute ileitis at day 9 p.i., NAP-treated mice exhibited less pronounced pro-inflammatory immune responses as indicated by lower numbers of intestinal mucosal T and B lymphocytes and lower interferon (IFN)-gamma concentrations in mesenteric lymph nodes. The NAP-induced anti-inflammatory effects were not restricted to the intestinal tract but could also be observed in extra-intestinal including systemic compartments, given that pro-inflammatory cytokines were lower in liver, kidney, and lung following NAP as compared to PLC application, whereas at day 9 p.i., colonic and serum interleukin (IL)-10 concentrations were higher in the former as compared to the latter. Remarkably, probiotic commensal bifidobacterial loads were higher in the ileal lumen of NAP as compared to PLC-treated mice with ileitis. Our findings thus further support that NAP might be regarded as future treatment option directed against intestinal inflammation.

引用

页码：34 / 40

页数：7

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