DEMONSTRATION OF A 7-NM RNP FIBER AS THE BASIC STRUCTURAL ELEMENT IN A PREMESSENGER RNP PARTICLE

被引：31

作者：

LONNROTH, A ^{[1
]}

ALEXCIEV, K ^{[1
]}

MEHLIN, H ^{[1
]}

WURTZ, T ^{[1
]}

SKOGLUND, U ^{[1
]}

DANEHOLT, B ^{[1
]}

机构：

[1] KAROLINSKA INST,MED NOBEL INST,DEPT MOLEC GENET,BOX 60400,S-10401 STOCKHOLM 60,SWEDEN

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 199卷 / 02期

关键词：

D O I：

10.1016/0014-4827(92)90437-D

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed after low salt treatment of isolated Balbiani ring granules. Moreover, the thin RNP fiber, 7 nm in diameter, can be revealed as the main structural element in Balbiani ring granules studied in situ in 3-D with electron microscope tomography. It is proposed that the thin RNP fiber consists of a premessenger RNA molecule coiled around a filamentous core of polymeric proteins, which has functional implications for processes such as assembly of RNP, intranuclear degradation of RNA, and delivery of RNA through the nuclear pores. © 1992.

引用

页码：292 / 296

页数：5

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