CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE GLUTAMATE-DEHYDROGENASE GENE, GDH, FROM CORYNEBACTERIUM-MELASSECOLA

被引：3
作者：
TAKEDA, Y
NAKAJYOH, Y
ISSHIKI, S
机构：
[1] Asahi Chemical Industry Company Ltd., Nobeoka, Miyazaki
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 69卷 / 06期
关键词：
D O I：
10.1016/0922-338X(90)90236-P
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A hybrid plasmid containing a fragment of the Corynebacterium melassecola chromosome cloned into pBR325 restored growth of glutamate auxotrophs of Escherichia coli strains that have mutations in the genes for glutamate dehydrogenase and glutamate synthase. A 3.1-kilobase pair region was shown by complementation analysis and enzyme measurements to carry the glutamate dehydrogenase gene, gdh. Glutamate dehydrogenase encoded by gdh carried on recombinant plasmids was elevated over 100-fold in E. coli cells. The gdh promoter was located by in vitro fusion to a promoter-deficient galK gene. © 1990.
引用
收藏
页码：317 / 321
页数：5
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