CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE GLUTAMATE-DEHYDROGENASE GENE, GDH, FROM CORYNEBACTERIUM-MELASSECOLA

被引：3
|
作者：
TAKEDA, Y
NAKAJYOH, Y
ISSHIKI, S
机构：
[1] Asahi Chemical Industry Company Ltd., Nobeoka, Miyazaki
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 69卷 / 06期
关键词：
D O I：
10.1016/0922-338X(90)90236-P
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A hybrid plasmid containing a fragment of the Corynebacterium melassecola chromosome cloned into pBR325 restored growth of glutamate auxotrophs of Escherichia coli strains that have mutations in the genes for glutamate dehydrogenase and glutamate synthase. A 3.1-kilobase pair region was shown by complementation analysis and enzyme measurements to carry the glutamate dehydrogenase gene, gdh. Glutamate dehydrogenase encoded by gdh carried on recombinant plasmids was elevated over 100-fold in E. coli cells. The gdh promoter was located by in vitro fusion to a promoter-deficient galK gene. © 1990.
引用
收藏
页码：317 / 321
页数：5
相关论文
共 50 条
  • [31] CLONING AND EXPRESSION OF A CLOSTRIDIUM-ACETOBUTYLICUM ALCOHOL-DEHYDROGENASE GENE IN ESCHERICHIA-COLI
    YOUNGLESON, JS
    SANTANGELO, JD
    JONES, DT
    WOODS, DR
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (03) : 676 - 682
  • [32] Molecular cloning, nucleotide sequence and expression in Escherichia coli of hyperthermophilic glutamate dehydrogenase gene from Thermococcus profundus
    Higuchi, S
    Kobayashi, T
    Kimura, K
    Horikoshi, K
    Kudo, T
    JOURNAL OF FERMENTATION AND BIOENGINEERING, 1997, 83 (05): : 405 - 411
  • [33] FORMATE DEHYDROGENASE FROM METHYLOTROPHIC BACTERIUM PSEUDOMONAS SP 101 - GENE CLONING AND EXPRESSION IN ESCHERICHIA-COLI
    TISHKOV, VI
    GALKIN, AG
    MARCHENKO, GN
    TSYGANKOV, YD
    EGOROV, AM
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1993, 18 : 201 - 207
  • [34] AMPLIFICATION OF RESPIRATORY NADH DEHYDROGENASE OF ESCHERICHIA-COLI BY GENE CLONING
    YOUNG, IG
    JAWOROWSKI, A
    POULIS, MI
    GENE, 1978, 4 (01) : 25 - 36
  • [35] CLONING AND EXPRESSION OF A PORCINE PRORELAXIN GENE IN ESCHERICHIA-COLI
    STEWART, AG
    RICHARDS, H
    ROBERTS, S
    WARWICK, J
    EDWARDS, K
    BELL, L
    SMITH, J
    DERBYSHIRE, R
    NUCLEIC ACIDS RESEARCH, 1983, 11 (19) : 6597 - 6609
  • [36] CLONING AND EXPRESSION OF THE FUMARATE REDUCTASE GENE OF ESCHERICHIA-COLI
    LOHMEIER, E
    HAGEN, DS
    DICKIE, P
    WEINER, JH
    CANADIAN JOURNAL OF BIOCHEMISTRY, 1981, 59 (03): : 158 - 164
  • [37] CLONING AND EXPRESSION OF THE PNEUMOCOCCAL NEURAMINIDASE GENE IN ESCHERICHIA-COLI
    BERRY, AM
    PATON, JC
    GLARE, EM
    HANSMAN, D
    CATCHESIDE, DEA
    GENE, 1988, 71 (02) : 299 - 305
  • [38] CLONING AND EXPRESSION OF THE PNEUMOCOCCAL AUTOLYSIN GENE IN ESCHERICHIA-COLI
    GARCIA, E
    GARCIA, JL
    RONDA, C
    GARCIA, P
    LOPEZ, R
    MOLECULAR & GENERAL GENETICS, 1985, 201 (02): : 225 - 230
  • [39] CLONING, CHARACTERIZATION, AND EXPRESSION OF THE DAPE GENE OF ESCHERICHIA-COLI
    BOUVIER, J
    RICHAUD, C
    HIGGINS, W
    BOGLER, O
    STRAGIER, P
    JOURNAL OF BACTERIOLOGY, 1992, 174 (16) : 5265 - 5271
  • [40] CLONING AND EXPRESSION OF A BACILLUS CELLULASE GENE IN ESCHERICHIA-COLI
    PARK, SH
    PACK, MY
    ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (12) : 725 - 728
12345