Wheat bran fermentation for the production of cellulase and xylanase by Aspergillus niger NFCCI 4113

被引:0
作者
Kumar, Bharti Amit [1 ]
Amit, Kumar [1 ,2 ]
Alok, Kumar [1 ]
Dharm, Dutt [1 ]
机构
[1] Indian Inst Technol Roorkee, Dept Paper Technol, Saharanpur Campus, Saharanpur 247001, India
[2] Debre Markos Univ, Coll Nat & Computat Sci, Dept Biotechnol, Debre Markos, Ethiopia
来源
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2018年 / 13卷 / 05期
关键词
Aspergillus niger; Wheat bran; Cellulases; Xylanases; Solid-state fermentation; beta-glucosidase;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Agricultural residues are the low-cost lignocellulosic materials that have potential to employ as the substrate for enzyme production under solid-state fermentation. Total eight solid substrates were examined for enzyme production; wheat bran was found to induce maximum endoglucanase (26.33 IU/gds), FPase (2.11 IU/gds) and beta-glucosidase (34.30 IU/gds by Aspergillus niger NFCCI 4113). After optimization of cultural parameters, Aspergillus niger NFCCI 4113 was found to produce maximum endoglucanase (55.26 IU/gds), FPase (4.64 IU/gds), xylanase (1175.76 IU/gds) and beta-glucosidase (71.30 IU/gds) at temperature (30 degrees C), incubation time (6 th day), pH (7.0) and moisture content (70%), using peptone (1.0%, w/v) as the nitrogen source and Tween-80 (0.10%, w/v) as the surfactant.
引用
收藏
页码:11 / 18
页数:8
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