DNA-PROTEIN COMPLEXES NATURAL TARGETS FOR DNA-HYDROLYZING ANTIBODIES

被引：36

作者：

GOLOLOBOV, GV

MIKHALAP, SV

STAROV, AV

KOLESNIKOV, AF

GABIBOV, AG

机构：

[1] RUSSIAN ACAD SCI,VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117901,RUSSIA

[2] UKRAINIAN ACAD SCI,KAVETSKY INST EXPTL PATHOL ONCOL & RADIOBIOL,KIEV,UKRAINE

[3] RUSSIAN ACAD MED SCI,INST VIROL,MOSCOW 109801,RUSSIA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 47卷 / 2-3期

关键词：

CATALYTIC ANTIBODIES; DNA; CROSSREACTIONS; NUCLEAR STAINING; LEUKEMIA; AIDS;

D O I：

10.1007/BF02787942

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sera of patients with different types of leukemia and acquired immune deficiency syndrome (AIDS) have been examined for the presence of the anti-DNA antibodies. DNA-hydrolyzing activity of antibodies was detected in the sera of patients with chronic lymphoid leukemia (CLL), pre-B-cell acute lymphoid leukemia (pre-B-All), acute myeloleukosis (AML), and AIDS in stages III and IV of the disease. In immunofluorescence tests, the DNA-hydrolyzing antibodies reacted preferentially with proliferating cell nuclei compared with resting cells. A 35-kDa factor was identified as the target for the DNA antibodies in the cell nuclei. The DNA-hydrolyzing antibody fraction from the serum of an AIDS patient crossreacted with HIV I virus proteins gp160, gp120, and p65.

引用

页码：305 / 315

页数：11

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