RAPID AND QUANTITATIVE ASSAY SYSTEM FOR MEASURING ANTIINFECTIOUS BURSAL DISEASE VIRUS-ANTIBODY USING MONOCLONAL-ANTIBODY BOUND TO POLYSTYRENE LATEX MICROSPHERES

被引：7
作者：
NAKAMURA, T
OTAKI, Y
LIN, Z
KATO, A
机构：
[1] Nippon Institute for Biological Science, Tokyo
来源：
AVIAN DISEASES | 1993年 / 37卷 / 03期
关键词：
D O I：
10.2307/1592030
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A monoclonal antibody (MAb) to infectious bursal disease virus (IBDV) that has virus-neutralizing activity was bound to polystyrene latex microspheres. The microspheres agglutinated with extracts of bursae from chickens infected with IBDV. Agglutination was inhibited in a competitive manner by adding serum obtained from IBDV-infected chickens. The level of agglutination-inhibition depended on the serum antibody titer against IBDV. The reaction was visually accomplished within 5 minutes. The titer of this rapid assay showed a close relationship with that of the virus-neutralization test.
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页码：786 / 792
页数：7
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