INDIGENOUS DOUBLE-STRANDED-RNA FROM PEPPER (CAPSICUM-ANNUUM)

被引：49

作者：

VALVERDE, RA

NAMETH, S

ABDALLHA, O

ALMUSA, O

DESJARDINS, P

DODDS, A

机构：

[1] OHIO STATE UNIV,DEPT PLANT PATHOL,COLUMBUS,OH 43210

[2] UNIV CALIF RIVERSIDE,DEPT PLANT PATHOL,RIVERSIDE,CA 92521

来源：

PLANT SCIENCE | 1990年 / 67卷 / 02期

关键词：

chloroplast; dsRNA; pepper;

D O I：

10.1016/0168-9452(90)90243-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A unique 12 kb linear double-stranded RNA (dsRNA) was detected in the chloroplast fraction of tissue extracts from pepper (Capsicum annuum). This dsRNA was detected in 10 out of 14 pepper cultivars tested. Molecular hybridization using cDNA to this dsRNA indicated that the 12 kb dsRNAs found in these pepper cultivars had nucleotide sequence similarity. This dsRNA did not hybridize to dsRNAs of similar size detected in bean and melon, nor to other dsRNAs found in non-virus inoculated plants. No evidence was obtained for a virus-like particle associated with this dsRNA. © 1990.

引用

页码：195 / 201

页数：7

共 17 条

[1]

ARCIDIACONO A, 1980, 7TH P EUR C EL MICR, V2, P516

[2]

Boccardo G., 1983, DOUBLE STRANDED RNA, P425

[3] ANALYSIS OF MITOCHONDRIAL-DNA, CHLOROPLAST DNA, AND DOUBLE-STRANDED-RNA IN FERTILE AND CYTOPLASMIC MALE-STERILE SUNFLOWER (HELIANTHUS-ANNUUS) [J].