ADSORPTION AND DESORPTION OF CELLULASE COMPONENTS DURING ENZYMATIC-HYDROLYSIS OF STEAMED SHIRAKAMBA (BETULA-PLATYPHYLLA SKATCHEV) WOOD

The adsorption behavior of Trichoderma viride cellulases on steamed shirakamba (Betula platyphylla Skatchev) wood was studied. Substrate/enzyme (S/E) ratio, hydrolysis time and saccharification extent as well as the presence or absence of lignin in the substrate were taken into consideration as factors affecting the enzyme adsorption. Filter paperase (FPase) components were adsorbed more selectively than other cellulase components on the substrate. The presence of lignin in the steamed hardwood tended to slow down the enzyme adsorption, but it did not appear to restrict the extent of hydrolysis of the carbohydrate moiety. The changes in the composition of the enzyme preparation during the course of hydrolysis were analyzed by fast protein liquid chromatography (FPLC). The irreversible adsorption of specific cellulase components was not observed in the prolonged hydrolysis of steamed shirakamba wood containing abundant lignin. The removal of lignin from steamed hardwood prior to hydrolysis is not always necessary for the recycling of cellulolytic enzymes.