Weight Loss and Melatonin Reduce Obesity-Induced Oxidative Damage in Rat Testis

被引：17
作者：
Atilgan, Dogan [1 ]
Parlaktas, Bekir S. [1 ]
Uluocak, Nihat [1 ]
Erdemir, Fikret [1 ]
Kilic, Sahin [1 ]
Erkorkmaz, Unal [2 ]
Ozyurt, Huseyin [3 ]
Markoc, Fatma [4 ]
机构：
[1] Gaziosmanpasa Univ, Fac Med, Dept Urol, TR-60100 Tokat, Turkey
[2] Sakarya Univ, Fac Med, Dept Biostat & Med Informat, TR-54100 Sakarya, Turkey
[3] Gaziosmanpasa Univ, Fac Med, Dept Biochem, TR-60100 Tokat, Turkey
[4] Gaziosmanpasa Univ, Fac Med, Dept Pathol, TR-60100 Tokat, Turkey
来源：
ADVANCES IN UROLOGY | 2013年 / 2013卷
关键词：
D O I：
10.1155/2013/836121
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
Aim. We aimed to evaluate the antioxidant effects of weight loss and melatonin on the obesity-induced oxidative damage in rat testes. Materials and Methods. 28 male Wistar albino rats were randomly divided into 4 groups, each consisting of 7 rats: control group (Group 1), obesity group (Group 2), obesity + MLT group (Group 3), and weight loss group (Group 4). Rats were weighed at the beginning and at the end of the study. Bilateral orchiectomy was performed and 5 cc blood samples were obtained from all of the rats. Superoxide dismutase (SOD), malondialdehyde (MDA), and protein carbonyl (PC) levels were analysed in the testicular tissues and serum. Spermatogenesis was evaluated with the Johnsen scoring system. Results. The testicular tissue and serum levels of MDA, PC, and SOD activity were increased in the obesity group in comparison to the sham operated group (P < 0.05). Weight loss and melatonin treatment ameliorated MDA, PC, and SOD levels in testicular tissue and serum significantly (P < 0.05). There was no significant difference between groups in terms of mean Johnsen score (P = 0.727). Conclusion. Experimentally created obesity caused oxidative stress and both melatonin and weight loss reduced oxidative stress parameters in rat testes.
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相关论文
共 35 条
[1]   Role of oxidants in male infertility: rationale, significance, and treatment [J].
Agarwal, A ;
Saleh, RA .
UROLOGIC CLINICS OF NORTH AMERICA, 2002, 29 (04) :817-+
[2]  
Agarwal A, 1994, CONTRACEPTION FERTIL, V22, P327
[3]   Is overweight a risk factor for reduced semen quality and altered serum sex hormone profile? [J].
Aggerholm, Anette S. ;
Thulstrup, Ane Marie ;
Toft, Gunnar ;
Ramlau-Hansen, Cecilia H. ;
Bonde, Jens Peter .
FERTILITY AND STERILITY, 2008, 90 (03) :619-626
[4]   Redox activity associated with the maturation and capacitation of mammalian spermatozoa [J].
Aitken, RJ ;
Ryan, AL ;
Baker, MA ;
McLaughlin, EA .
FREE RADICAL BIOLOGY AND MEDICINE, 2004, 36 (08) :994-1010
[5]   Increased seminal reactive oxygen species levels in patients with varicoceles correlate with varicocele grade but not with testis size [J].
Allamaneni, SSR ;
Naughton, CK ;
Sharma, RK ;
Thomas, AJ ;
Agarwal, A .
FERTILITY AND STERILITY, 2004, 82 (06) :1684-1686
[6]   Body mass index in relation to semen quality, sperm DNA integrity, and serum reproductive hormone levels among men attending an infertility clinic [J].
Chavarro, Jorge E. ;
Toth, Thomas L. ;
Wright, Diane L. ;
Meeker, John D. ;
Hauser, Russ .
FERTILITY AND STERILITY, 2010, 93 (07) :2222-2231
[7]   The suppressive effect of dietary restriction and weight loss in the obese on the generation of reactive oxygen species by leukocytes, lipid peroxidation, and protein carbonylation [J].
Dandona, P ;
Mohanty, P ;
Ghanim, H ;
Aljada, A ;
Browne, R ;
Hamouda, W ;
Prabhala, A ;
Afzal, A ;
Garg, R .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2001, 86 (01) :355-362
[8]   Healthy overweight male partners of subfertile couples should not worry about their semen quality [J].
Duits, Floor H. ;
van Wely, Madelon ;
van der Veen, Fulco ;
Gianotten, Judith .
FERTILITY AND STERILITY, 2010, 94 (04) :1356-1359
[9]  
Egwurugwu J N, 2011, Niger J Physiol Sci, V26, P29
[10]  
Erdemir F, 2012, ACTAS UROL ESP, V36, P153, DOI [10.1016/j.acuro.2011.06.019, 10.1016/j.acuroe.2012.05.003]
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