EXPRESSION OF RICIN-B CHAIN IN SPODOPTERA-FRUGIPERDA

被引：22

作者：

FRANKEL, A ^{[1
]}

ROBERTS, H ^{[1
]}

AFRIN, L ^{[1
]}

VESELY, J ^{[1
]}

WILLINGHAM, M ^{[1
]}

机构：

[1] MED UNIV S CAROLINA,DEPT PATHOL,CHARLESTON,SC 29425

来源：

BIOCHEMICAL JOURNAL | 1994年 / 303卷

关键词：

D O I：

10.1042/bj3030787

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA encoding ricin B chain was derived from preproricin genomic DNA and ligated into the baculovirus transfer vector, pAcGP67A. Co-transfection of Spodoptera frugiperda Sf9 cells with BaculoGold DNA was followed by limiting dilution purification of recombinant baculovirus. Infection of SF9 cells at a multiplicity of infection of 1 in the presence of 25 mM lactose produced 3 mg/1 of soluble, glycosylated, immunoreactive with monoclonal and polyclonal antibodies to ricin B chain. The recombinant ricin B chain had similar lectin-binding activity to plant ricin B chain. The recombinant protein reassociated with ricin toxin. A chain similarly to ricin toxin B chain and the recombinant heterodimers had similar cell cytotoxicity to ricin.

引用

页码：787 / 794

页数：8

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