CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDY OF THE NICKEL-BINDING PROTEIN NIKA OF ESCHERICHIA-COLI

被引：14
作者：
CHARON, MH
WU, LF
PIRAS, C
DEPINA, K
MANDRANDBERTHELOT, MA
FONTECILLACAMPS, JC
机构：
[1] CNRS,CEA,INST BIOL STRUCT JP EBEL,CRISTALLOG & CRISTALLOGENESE PROT LAB,F-38027 GRENOBLE 1,FRANCE
[2] INST NATL SCI APPL,GENET MOLEC MICROORGANISMS & INTERACT CELLULAIRES,CNRS,URA 1486,F-69621 VILLEURBANNE,FRANCE
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 243卷 / 02期
关键词：
NICKEL CHEMOTAXIS; CRYSTALLIZATION; SOLUTE-BINDING PROTEINS;
D O I：
10.1006/jmbi.1994.1661
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NikA, a periplasmic nickel-binding protein involved in nickel-repellent chemotaxis has been crystallized. The crystals are hexagonal with space group P6(2) (or its enantiomorph) with a = 160.3 Angstrom and c = 138.4 Angstrom and they diffract to at least 3.0 Angstrom resolution in the laboratory NikA presents sequence homology with several periplasmic solute-binding proteins from Gram-negative bacteria.
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页码：353 / 355
页数：3
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