DETERMINATION OF AFLATOXIN Q(1) IN URINE BY AUTOMATED IMMUNOAFFINITY COLUMN CLEANUP AND LIQUID-CHROMATOGRAPHY

被引：13

作者：

KUSSAK, A ^{[1
]}

ANDERSSON, B ^{[1
]}

ANDERSSON, K ^{[1
]}

机构：

[1] NATL INST OCCUPAT HLTH,DIV ANALYT CHEM,S-90713 UMEA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00123-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic system with an automated clean-up procedure for aflatoxin Q(1) in human urine is described. The samples were cleaned up by using immunoaffinity columns originally designed for aflatoxin M(1). The chromatographic system was a C-18 column with an acidic mobile phase of acetonitrile-water containing potassium bromide. Fluorescence detection (365/440 nm) of aflatoxin Q(1) was enhanced by addition of bromine, using post-column derivatization, which was studied by factorial designs. Average recovery of aflatoxin Q(1) in spiked 10-ml urine samples was 88% (R.S.D. = 6.4%) at a level of 50 pg/ml. The determination limit was 49.5 pg/ml urine.

引用

页码：329 / 334

页数：6

共 18 条

[1] DETERMINATION OF EXPOSURE TO AFLATOXINS AMONG DANISH WORKERS IN ANIMAL-FEED PRODUCTION THROUGH THE ANALYSIS OF AFLATOXIN-B1 ADDUCTS TO SERUM-ALBUMIN [J].

AUTRUP, JL ;

SCHMIDT, J ;

SEREMET, T ;

AUTRUP, H .

SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH, 1991, 17 (06) :436-440

[2]

BETINA V, 1989, BIOACTIVE MOL, V9

[3]

CARLSON R, 1992, DESIGN OPTIMIZATION

[4] TANDEM CHROMATOGRAPHIC-IMMUNOLOGICAL ANALYSES [J].

DEFRUTOS, M ;

REGNIER, FE .

ANALYTICAL CHEMISTRY, 1993, 65 (01) :A17-&

[5]

EATON DL, 1990, 1988 P S MICR TOX FO

[6] DETERMINATION OF AFLATOXIN-M1 USING A DIALYSIS-BASED IMMUNOAFFINITY SAMPLE PRETREATMENT SYSTEM COUPLED ONLINE TO LIQUID-CHROMATOGRAPHY - REUSABLE IMMUNOAFFINITY COLUMNS [J].

FARJAM, A ;

VANDEMERBEL, NC ;

NIEMAN, AA ;

LINGEMAN, H ;

BRINKMAN, UAT .

JOURNAL OF CHROMATOGRAPHY, 1992, 589 (1-2) :141-149

[7]

GROOPMAN JD, 1992, CANCER EPIDEM BIOMAR, V1, P221

[8] DETERMINATION OF AFLATOXINS IN CATTLE FEED BY LIQUID-CHROMATOGRAPHY AND POSTCOLUMN DERIVATIZATION WITH ELECTROCHEMICALLY GENERATED BROMINE [J].

KOK, WT ;

VANNEER, TCH ;

TRAAG, WA ;

TUINSTRA, LGMT .

JOURNAL OF CHROMATOGRAPHY, 1986, 367 (01) :231-236

[9] AUTOMATED SAMPLE CLEANUP WITH SOLID-PHASE EXTRACTION FOR THE DETERMINATION OF AFLATOXINS IN URINE BY LIQUID-CHROMATOGRAPHY [J].

KUSSAK, A ;

ANDERSSON, B ;

ANDERSSON, K .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 616 (02) :235-241

[10] METABOLISM OF AFLATOXIN-B1 IN THE BOVINE OLFACTORY MUCOSA [J].

LARSSON, P ;

PETTERSSON, H ;

TJALVE, H .

CARCINOGENESIS, 1989, 10 (06) :1113-1118

← 1 2 →