SIMULTANEOUS SOLID-PHASE EXTRACTION AND CHROMATOGRAPHIC ANALYSIS OF MORPHINE AND HYDROMORPHONE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：28
作者：
BOUQUILLON, AI
FREEMAN, D
MOULIN, DE
机构：
[1] UNIV WESTERN ONTARIO,VICTORIA HOSP,DEPT CLIN NEUROL SCI,PAIN RES PROGRAM,375 SOUTH ST,LONDON N6A 4G5,ONTARIO,CANADA
[2] UNIV WESTERN ONTARIO,VICTORIA HOSP,DEPT MED CLIN PHARMACOL,LONDON N6A 4G5,ONTARIO,CANADA
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 577卷 / 02期
关键词：
D O I：
10.1016/0378-4347(92)80258-R
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
High-performance liquid chromatography has become an important analytical tool for the quantitation of opioid drugs. Using solid-phase extraction and coulometric electrochemical detection. we have developed a chromatographic method for the simultaneous measurement of morphine and hydromorphone which is both sensitive and specific. Using 1 ml of plasma, intra-assay and inter-assay data show that the detection limit for accurate quantitation of these compounds is about 1.2 ng/ml (coefficient of variation 11.6%) for morphine and 2.5 ng/ml (coefficient of variation 10.5%) for hydromorphone. The method is simple and readily adaptable to most pharmacokinetic studies and toxic screens involving these drugs.
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页码：354 / 357
页数：4
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