DETECTION OF A POLYMORPHISM WITHIN THE PEPSINOGEN-C GENE WITH PCR - CONSTRUCTION OF A LINKAGE MAP AROUND PGC FROM 6P11-6P21.3

被引：5

作者：

BOWCOCK, AM

AZUMA, T

BARNES, RI

WU, SH

BELL, GI

TAGGART, RT

机构：

[1] KYOTO PREFECTURAL UNIV,DEPT PREVENT MED,KYOTO 606,JAPAN

[2] UNIV CHICAGO,HOWARD HUGHES MED INST,CHICAGO,IL 60637

[3] WAYNE STATE UNIV,SCH MED,DEPT MOLEC BIOL & GENET,DETROIT,MI 48201

来源：

GENOMICS | 1992年 / 14卷 / 02期

关键词：

D O I：

10.1016/S0888-7543(05)80232-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An insertion/deletion polymorphism between exons 7 and 8 of the pepsinogen C gene (PGC), previously detectable with Southern analysis, was formatted for detection with PCR. Alleles were rapidly typed by UV irradiation of ethidium bromide-stained agarose gels. Whereas Southern analysis revealed two alleles, the smaller fragments generated with PCR allowed the resolution of three alleles that were previous, scored as a single allele and increased the heterozygosity of the system from 0.20 to 0.53. After a set of reference families was genotyped with the PCR-based polymorphism, a linkage map around the PGC gene on chromosome 6 was constructed. This included the HLA cluster and the highly informative D6S223 locus. PGC lies 22 cM proximal to HLA-DPB and between D6S5 and D6S4 at distances of 4.5 and 13.1 cM, respectively. © 1992 Academic Press, Inc. All rights reserved.

引用

页码：398 / 402

页数：5

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