Determination of azathioprine in bulk and pharmaceutical dosage form by HPTLC

被引：5

作者：

Jain, Pritam S. ^{[1
]}

Thakre, Pradip ^{[1
]}

Chaudhari, Amar J. ^{[1
]}

Chavhan, Manish L. ^{[1
]}

Surana, Sanjay J. ^{[1
]}

机构：

[1] RC Patel Inst Pharmaceut Educ & Res, Dept Pharmaceut Chem, Shirpur, Maharashtra, India

来源：

JOURNAL OF PHARMACY AND BIOALLIED SCIENCES | 2012年 / 4卷 / 04期

关键词：

Azathioprine; HPTLC; validation;

D O I：

10.4103/0975-7406.103263

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Introduction: An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated. Materials and Methods: The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done by densitometric scanning at 300nm. Results: Response was a linear function of Azathioprine concentration in the range of 200-1200 ng/band. The limit of detection and quantification for Azathioprine were 18.58 and 59.14 ng/band, respectively. Average recovery was 100.1% which shows that the method was free from interference from excipients present in the formulation. Conclusion: The established method enabled accurate, precise, and rapid analysis of Azathioprine in bulk as well as pharmaceutical formulation.

引用

页码：318 / 321

页数：4

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