OPTIMIZATION OF THE DETERMINATION OF AMINO-ACIDS IN PARENTERAL SOLUTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN DERIVATIZATION USING 9-FLUORENYLMETHYL CHLOROFORMATE

An automatic precolumn derivatization procedure is presented for the determination of amino acids in parenteral solutions by reversed-phase high-performance liquid chromatography. The fluorenylmethyl chloroformate was used as the reagent for derivatization. The optimum conditions for the derivatization reaction, separation and detection were investigated from reproducibility and linearity data. This method has the advantage of automatic precolumn derivatization, a shorter analysis time with optimum separation and high reproducibility of retention times and peak areas for all amino acids with both UV and fluorescence detection.