OPTIMIZATION OF THE DETERMINATION OF AMINO-ACIDS IN PARENTERAL SOLUTIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRECOLUMN DERIVATIZATION USING 9-FLUORENYLMETHYL CHLOROFORMATE

被引：24
作者：
CARRATU, B
BONIGLIA, C
BELLOMONTE, G
机构：
[1] Istituto Superiore di Sanità, Laboratorio Alimenti, 00161 Rome
来源：
JOURNAL OF CHROMATOGRAPHY A | 1995年 / 708卷 / 02期
关键词：
D O I：
10.1016/0021-9673(95)00409-G
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An automatic precolumn derivatization procedure is presented for the determination of amino acids in parenteral solutions by reversed-phase high-performance liquid chromatography. The fluorenylmethyl chloroformate was used as the reagent for derivatization. The optimum conditions for the derivatization reaction, separation and detection were investigated from reproducibility and linearity data. This method has the advantage of automatic precolumn derivatization, a shorter analysis time with optimum separation and high reproducibility of retention times and peak areas for all amino acids with both UV and fluorescence detection.
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页码：203 / 208
页数：6
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