MULTISTEP DNA CLEAVAGE IN RAT-LIVER NUCLEI IS INHIBITED BY THIOL REACTIVE AGENTS

被引：19

作者：

CAIN, K

INAYATHUSSAIN, SH

KOKILEVA, L

COHEN, GM

机构：

[1] MRC Toxicology Unit, University of Leicester, Leicester, LE1 9HN, Hodgkin Building, Lancaster Rd

来源：

FEBS LETTERS | 1995年 / 358卷 / 03期

关键词：

APOPTOSIS; MULTISTEP DNA CLEAVAGE; CRITICAL THIOL;

D O I：

10.1016/0014-5793(94)01436-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA fragmentation in isolated rat liver nuclei is a Mg2+-dependent, multi-step process which is potentiated by Ca2+ and cleaves the DNA into greater than or equal to 700, 200-300 and 30-50 kilobase pair (kbp) fragments, prior to internucleosomal cleavage by Ca2+/ Mg2+-dependent endonuclease(s). We now show that Cd2+, Hg2+, dichloroisocoumarin (DCI, a serine protease inhibitor) and N- ethylmaleimide (NEM) block both Mg2+ and Ca2+/Mg2+-dependent processes. Inhibition of DNA cleavage produced an increase in the size of the DNA fragments, from mono-/oligonucleosomes to 30-50, 200-300, greater than or equal to 700 kbp and finally to intact DNA. NEM and DCI inhibition was blocked by dithiothreitol, and it is proposed that a critical thiol(s) is involved in the DNA cleavage reactions which are a feature of the apoptotic process.

引用

页码：255 / 261

页数：7

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[4] DNA FRAGMENTATION INTO 200-250-KILOBASE AND/OR 30-50-KILOBASE PAIR FRAGMENTS IN RAT-LIVER NUCLEI IS STIMULATED BY MG2+ ALONE AND CA2+/MG2+ BUT NOT BY CA2+ ALONE [J].