AN EXTRACHROMOSOMAL FRAGMENT OF TELOMERIC DNA IN WHEAT

被引：14

作者：

BUCHOLC, M ^{[1
]}

BUCHOWICZ, J ^{[1
]}

机构：

[1] POLISH ACAD SCI,INST BIOCHEM & BIOPHYS,PL-02106 WARSAW,POLAND

来源：

PLANT MOLECULAR BIOLOGY | 1995年 / 27卷 / 02期

关键词：

BUOYANT SEPARATION; CLONING; EXTRACHROMOSOMAL DNA; GERMINATION; TELOMERE; TRITICUM AESTIVUM;

D O I：

10.1007/BF00020198

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure developed originally for selective extraction of viral (extrachromosomal) DNA from virus-infected mammalian cells was applied to cell nuclei isolated from uninfected wheat embryos. The resulting nuclear extrachromosomal DNA (exDNA) was enriched for telomere-type sequences by isopycnic centrifugation and inserted into the Sma I site of pUC119. A cloned DNA fragment (241 bp) was found to consist primarily of tandemly repeated heptamere units of the same sequence (5'-CCCTAAA-3') that is known to predominate in telomeric DNA of Arabidopsis thaliana. Hybridization experiments indicate that extrachromosomal telomeric repeats are abundant in resting embryos and disappear rapidly during germination.

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收藏

页码：435 / 439

页数：5

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