EFFECT OF CARBON-DIOXIDE AND ETHYLENE ON BERBERINE PRODUCTION AND CELL BROWNING IN THALICTRUM-MINUS CELL-CULTURES

被引：8

作者：

KOBAYASHI, Y ^{[1
]}

FUKUI, H ^{[1
]}

TABATA, M ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,KYOTO 606,JAPAN

来源：

PLANT CELL REPORTS | 1991年 / 9卷 / 09期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cultured cells of Thalictrum minus L. (Ranunculaceae), transferred from culture flasks to a bubble column bioreactor, produced little berberine and turned dark brown, even when supplied with sufficient oxygen. This phenomenon was ascribed to the removal of CO2 from the culture medium by bubbling air, and could be reproduced in flask cultures artificially deprived of CO2. The induction of cell browning by exogenously administered ethylene suggested that CO2 probably acts antagonistically against endogenously generated C2H4. The physiological damage caused by forced aeration could be prevented by adding 2 % CO2 to the air in the bioreactor.

引用

页码：496 / 499

页数：4

共 21 条

[11] POLYPHENOL OXIDASES IN PLANTS [J].