CLONING OF A MOUSE RABPHILIN-3A EXPRESSED IN HORMONE-SECRETING CELLS

被引：27

作者：

INAGAKI, N ^{[1
]}

MIZUTA, M ^{[1
]}

SEINO, S ^{[1
]}

机构：

[1] CHIBA UNIV,SCH MED,CTR BIOMED SCI,DIV MOLEC MED,CHUO KU,CHIBA 260,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 116卷 / 02期

关键词：

C2; DOMAIN; EXOCYTOSIS; RAB3A; SYNAPTOTAGMIN; VESICLE PROTEIN;

D O I：

10.1093/oxfordjournals.jbchem.a124512

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rab3A, a ras p21-related small GTP-binding protein, is implicated in the exocytosis of neurotransmitters. Recently, Rabphilin-3A, a putative target protein for Rab3A, was identified and its cDNA has been cloned from bovine brain. In this study, we isolated a cDNA encoding a mouse Rabphilin-3A homolog from the insulin-secreting cell line, MIN6. Mouse Rabphilin-3A is a protein of 681 amino acids exhibiting overall 88.5% identity with bovine Rabphilin-3A. The amino acid identity between mouse and bovine Rabphilin-3A is highest in their carboxyl-terminal halves (97.8% identity) and amino-termini (93.0% identity), which contain the region of the two internal repeats homologous to the regulatory domain (C-2 domain) of protein kinase C and the putative Rab3A-binding region, respectively. RNA blot analysis revealed that Rabphilin-3A mRNA is expressed in endocrine and hormone-secreting clonal cells, including rat adrenal glands, MIN6, the hamster insulin-secreting cell line, HIT-T15, and the rat catecholamine-secreting cell line, PC12, as well as rat brain. These results suggest that Rabphilin-3A might be involved in the exocytosis of secretory vesicles in hormone-secreting cells as well as in neurons.

引用

页码：239 / 242

页数：4

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