Genotyping of Toxoplasma gondii Isolated from cat Feces in Songkhla, Southern Thailand

被引:11
|
作者
Chemoh, Waenurama [1 ]
Sawangjaroen, Nongyao [2 ]
Nissapatorn, Veeranoot [3 ,4 ]
Sermwittayawong, Natthawan [2 ]
机构
[1] Princess Naradhiwas Univ, Fac Med, Dept Microbiol, Narathiwat 96000, Thailand
[2] Prince Songkla Univ, Fac Sci, Dept Microbiol, Hat Yai 90110, Songkhla, Thailand
[3] Walailak Univ, Res Excellence Ctr Innovat & Hlth Prod RECIHP, Nakhon Si Thammarat 80161, Thailand
[4] Walailak Univ, Sch Allied Hlth Sci, Nakhon Si Thammarat 80161, Thailand
来源
VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS | 2018年 / 13卷
关键词
Atypical T. gondii; Genotyping; PCR-RFLP; Toxoplasma oocyst;
D O I
10.1016/j.vprsr.2018.04.005
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The genotype of Toxoplasma gondii has been extensively studied to determine whether its characteristics may influence the course of toxoplasmosis. Recent genotyping studies in South America have found Toxoplasma hybrid isolates or atypical strains which were more virulent and highly diverse than North American and European species. Although, the high seroprevalence of Toxoplasma infection among pregnant women and HIV-infected patients from Songklanagarind Hospital, Songkhla, Southern Thailand has been previously reported, there is no reported study of human genotyping data on T. gondii strains. This is the first genetic typing of T. gondii isolates obtained from naturally infected cats in Thailand. Five chromosomal loci were analyzed by a nested PCR-RFLP technique to determine the genotype of 13 T. gondii isolates from cat feces gathered in the South of Thailand. The PCR-RFLP patterns of SAG1, SAG2-new, SAG3, BTUB and GRA6 markers resulted in five diverse genotypes: the type I (one isolate), type III (two isolates), type II or type III (one isolate), recombinant genotypes (two isolates) and atypical genotypes (two isolates). The presence of unusual genotypes may lead to new virulent traits associated with more severe forms of human Toxoplasma infections. More evaluations are needed before conclusions could be made as to whether the oocysts from cat feces play an important role in the severity of Toxoplasma infections.
引用
收藏
页码:105 / 109
页数:5
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