THE SYNTHETIC [TYR(5,12),LYS(7)]-POLYPHEMUSIN-II PEPTIDE (T22) BINDS TO THE CD4 CELL-SURFACE MOLECULE

被引：14

作者：

WEEKS, BS

NOMIZU, M

OTAKA, A

WESTON, CA

OKUSU, A

TAMAMURA, H

YAMAMOTO, N

FUJII, N

机构：

[1] NIDR,DEV BIOL LAB,BETHESDA,MD 20892

[2] KYOTO UNIV,FAC PHARMACEUT SCI,SAKYO KU,KYOTO 606,JAPAN

[3] HAMILTON COLL,DEPT BIOL,CLINTON,NY 13323

[4] TOKYO MED & DENT UNIV,SCH MED,DEPT MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2510

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The [Tyr(5,12), Lys(7)]-polyphemusin II peptide (T22) inhibits HIV-1 replication in lymphocytes. The mechanism of T22 inhibition of HIV-1 replication may involve T22 competition with HIV-1 for attachment sites on the plasma membrane of targeted cells. Here we find that the T22 peptide binds to the CD4 molecule in affinity columns. We also find that antiserum to CD4 inhibits cell attachment to T22. Further CD4+ transfected cells attach to T22 while their parental cells which do not express CD4 do not attach to T22. These data demonstrate that T22 binds to the CD4 molecule and supports the hypothesis that T22 inhibits HIV-1 replication by binding to the cell surface CD4 molecule and inhibiting uptake of the virus. (C) 1995 Academic Press, Inc.

引用

页码：626 / 631

页数：6

共 17 条

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