A REACTIVE MACROMOLECULAR MATRIX FOR PROTEIN IMMOBILIZATION ON A GOLD SURFACE - APPLICATION IN SURFACE-PLASMON RESONANCE

被引：18

作者：

MILLOT, MC ^{[1
]}

MARTIN, F ^{[1
]}

BOUSQUET, D ^{[1
]}

SEBILLE, B ^{[1
]}

LEVY, Y ^{[1
]}

机构：

[1] INST OPT,F-91403 ORSAY,FRANCE

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1995年 / 29卷 / 1-3期

关键词：

SURFACE PLASMON RESONANCE SENSORS; IMMUNOSENSORS; CYSTEAMINE; REACTIVE POLYMER; RABBIT IMMUNOGLOBULIN G;

D O I：

10.1016/0925-4005(95)01693-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reactive copolymer bearing functions able to react at room temperature with amino groups has been immobilized on a cysteamine-coated gold surface. Polymer immobilization, covalent coupling of rabbit immunoglobulin G and antigen-antibody reactions were studied using a surface plasmon resonance (SPR) technique. The effect of a protein A layer on the antigen-antibody reaction was examined.

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页码：268 / 273

页数：6

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