MAPPING OF A SEROTYPE-SPECIFIC EPITOPE OF THE MAJOR CAPSID PROTEIN VP2 OF INFECTIOUS PANCREATIC NECROSIS VIRUS

被引：19

作者：

LIAO, L ^{[1
]}

DOBOS, P ^{[1
]}

机构：

[1] UNIV GUELPH, COLL BIOL SCI, DEPT MICROBIOL, GUELPH, ON N1G 2W1, CANADA

来源：

VIROLOGY | 1995年 / 209卷 / 02期

关键词：

D O I：

10.1006/viro.1995.1304

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A serotype-specific, conformational epitope of VP2 of infectious pancreatic necrosis virus (IPNV) (Jasper), has been mapped by restriction enzyme site-specific deletions of cloned viral cDNA. Subclones encoding fragments of VP2 were expressed in Escherichia coli followed by polyacrylamide gel electrophoresis and Western blot analysis. The epitope (between amino acid residues 242 and 325) reacted with monoclonal anti-VP2 antibodies that neutralized the homologous (Jasper), but not the heterologous (Sp) serotype of IPNV. (C) 1995 Academic Press, Inc.

引用

页码：684 / 687

页数：4

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