DIFFERENTIAL GENE-EXPRESSION IN AZOSPIRILLUM SPP BY PLANT-ROOT EXUDATES - ANALYSIS OF PROTEIN PROFILES BY 2-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS

被引：0

作者：

VANBASTELAERE, E ^{[1
]}

DEMOT, R ^{[1
]}

MICHIELS, K ^{[1
]}

VANDERLEYDEN, J ^{[1
]}

机构：

[1] UNIV LEUVEN,FA JANSSENS LAB,W CROYLAAN 42,B-3001 HEVERLEE,BELGIUM

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 112卷 / 03期

关键词：

AZOSPIRILLUM; ROOT EXUDATES; WHEAT; MAIZE; OUTER MEMBRANE;

D O I：

10.1016/0378-1097(93)90622-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two-dimensional polyacrylamide gel electrophoresis of total proteins of Azospirillum brasilense Sp7 showed that the bacteria respond to the presence of plant root exudates by induction of some proteins. High-level induction of a 40-kDa acidic protein was demonstrated by Coomassie brilliant blue staining and Western blotting, using a specific polyclonal antiserum. This protein is also observed in A. lipoferum, but not in A. halopraeferens and A. irakense. In A. brasilense, the 40-kDa protein was induced by exudates from wheat, maize, bean and alfalfa. However, only maize exudates stimulated production of this protein in A. lipoferum.

引用

页码：335 / 342

页数：8

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