ANION TRANSPORTER - HIGHLY CELL-TYPE-SPECIFIC EXPRESSION OF DISTINCT POLYPEPTIDES AND TRANSCRIPTS IN ERYTHROID AND NONERYTHROID CELLS

Affinity-purified antibodies and c[complementary]DNA probes specific for the chicken erythrocyte anion transporter (also referred to as band 3) were used to demonstrate that this protein is expressed in a highly cell-type-specific manner in the avian kidney. Indirect immunofluorescence analysis indicates that this polypeptide is present in only a small subset of total kidney cells and is predominantly localized to the proximal convoluted tubule of this organ. Chicken erythrocytes synthesize and accumulate 2 structurally and serologically related band 3 polypeptides. The polypeptide that accumulates in kidney membranes has an apparent MW greater than either of its erythroid counterparts. This diversity is also reflected at the RNA level, as the single band 3 mRNA species detected during various stages of erythroid development is distinct in size from that found in kidney cells. Genomic DNA blot analysis suggests that both the erythroid and kidney band 3 RNA arise from a single gene. Of the adult tissues examined that are known to express ankyrin and spectrin polypeptides, only kidney accumulates detectable levels of the band 3 mRNA and polypeptide. A subset of kidney cell use an anion transport mechanism analogous to that of erythrocytes and that band 3 is expressed in a noncoordinate manner with other components of the erythroid membrane skeleton in nonerythroid cells.