ONTOGENY OF BASIC FIBROBLAST GROWTH-FACTOR BINDING-SITES IN MOUSE OCULAR-TISSUES

被引:54
作者
FAYEIN, NA [1 ]
COURTOIS, Y [1 ]
JEANNY, JC [1 ]
机构
[1] CNRS, UA 630,INSERM,U118,UNITE RECH GERONTOL, 29 RUE WILHEM, F-75016 PARIS, FRANCE
关键词
D O I
10.1016/0014-4827(90)90280-N
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodinated bFGF. One specific FGF binding site (KD = 2.5 nM) is colocalized with heparan sulfate proteoglycans of the basement membranes and is heparitinase sensitive. It first appears at Day 9 around the neural tube, the optic vesicles, and below the head ectoderm and by Day 14 of embryonic development is found in all basement membranes of the eye. At Day 16, very intensely labeled patches appear, corresponding to mast cells which have been characterized by metachromatic staining of their heparin-rich granulations with toluidine blue. In addition to the latter binding, we have also observed a general diffuse distribution of silver grains on all tissues and preferentially in the ecto- and neuroectodermic tissues. From Days 17-18, there is heterogeneous labeling inside the retina, localized in the pigmented epithelium and in three different layers colocalized with the inner and outer plexiform layers and with the inner segments of the photoreceptors. This binding is heparitinase resistant but N-glycanase sensitive and may represent a second specific binding site corresponding to cellular FGF receptors (KD = 280 pM). Both types of binding patterns observed suggest a significant role for bFGF in eye development and physiology. © 1990.
引用
收藏
页码:75 / 88
页数:14
相关论文
共 59 条
[1]  
ANDERSON DH, 1981, INVEST OPHTH VIS SCI, V21, P10
[2]   BASIC FIBROBLAST GROWTH-FACTOR PREVENTS DEATH OF LESIONED CHOLINERGIC NEURONS INVIVO [J].
ANDERSON, KJ ;
DAM, D ;
LEE, S ;
COTMAN, CW .
NATURE, 1988, 332 (6162) :360-361
[3]   MORPHOLOGICAL-CHANGES AND GROWTH STIMULATION OF BOVINE EPITHELIAL LENS CELLS BY A RETINAL EXTRACT INVITRO [J].
ARRUTI, C ;
COURTOIS, Y .
EXPERIMENTAL CELL RESEARCH, 1978, 117 (02) :283-292
[4]   AN EYE-DERIVED GROWTH-FACTOR REGULATES EPITHELIAL-CELL PROLIFERATION IN THE CULTURED LENS [J].
ARRUTI, C ;
CIRILLO, A ;
COURTOIS, Y .
DIFFERENTIATION, 1985, 28 (03) :286-290
[5]   AMINO SUGAR-CONTAINING COMPOUNDS OF RETINA .1. ISOLATION AND IDENTIFICATION [J].
BACH, G ;
BERMAN, ER .
BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 252 (03) :453-&
[6]   RETINA-DERIVED AND EYE-DERIVED ENDOTHELIAL-CELL GROWTH-FACTORS - PARTIAL MOLECULAR CHARACTERIZATION AND IDENTITY WITH ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS [J].
BAIRD, A ;
ESCH, F ;
GOSPODAROWICZ, D ;
GUILLEMIN, R .
BIOCHEMISTRY, 1985, 24 (27) :7855-7860
[7]   CELL-DIFFERENTIATION AND PATTERN-FORMATION IN THE DEVELOPING MAMMALIAN RETINA [J].
BARNSTABLE, CJ ;
BLUM, AS ;
DEVOTO, SH ;
HICKS, D ;
MORABITO, MA ;
SPARROW, JR ;
TREISMAN, JE .
NEUROSCIENCE RESEARCH, 1988, :S27-S41
[8]   PURIFICATION, CHARACTERIZATION, AND BIOLOGICAL PROPERTIES OF THE EYE-DERIVED GROWTH-FACTOR FROM RETINA - ANALOGIES WITH BRAIN-DERIVED GROWTH-FACTOR [J].
BARRITAULT, D ;
PLOUET, J ;
COURTY, J ;
COURTOIS, Y .
JOURNAL OF NEUROSCIENCE RESEARCH, 1982, 8 (2-3) :477-490
[9]   ACID MUCOPOLYSACCHARIDES OF CATTLE RETINA [J].
BERMAN, ER ;
BACH, G .
BIOCHEMICAL JOURNAL, 1968, 108 (01) :75-&
[10]   BINDING, INTERNALIZATION, AND DEGRADATION OF BASIC FIBROBLAST GROWTH-FACTOR IN HUMAN MICROVASCULAR ENDOTHELIAL-CELLS [J].
BIKFALVI, A ;
DUPUY, E ;
INYANG, AL ;
FAYEIN, N ;
LESECHE, G ;
COURTOIS, Y ;
TOBELEM, G .
EXPERIMENTAL CELL RESEARCH, 1989, 181 (01) :75-84