LOCALIZATION OF PEPTIDASES IN LACTOCOCCI

被引：75
作者：
TAN, PST
CHAPOTCHARTIER, MP
POS, KM
ROUSSEAU, M
BOQUIEN, CY
GRIPON, JC
KONINGS, WN
机构：
[1] UNIV GRONINGEN,DEPT MICROBIOL,KERKLAAN 30,9751 NN HAREN,NETHERLANDS
[2] INRA,GENIE PROCEDES BIOTECHNOL AGROALIMENTAIRES LAB,F-7850 THIVERVAL GRIGNON,FRANCE
[3] INRA,RECH LAITIERES STN,F-78350 JOUY EN JOSAS,FRANCE
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 01期
关键词：
D O I：
10.1128/AEM.58.1.285-290.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The localization of two aminopeptidases, an X-prolyl-dipeptidyl aminopeptidase, an endopeptidase, and a tripeptidase in Lactococcus lactis was studied. Polyclonal antibodies raised against each purified peptidase are specific and do not cross-react with other peptidases. Experiments were performed by immunoblotting after cell fractionation and by electron microscopy of immunogold-labeled peptidases. All peptidases were found to be intracellular. However, immunogold studies showed a peripheral labeling of the X-prolyl-dipeptidyl aminopeptidase, the tripeptidase, and the endopeptidase. This peripheral location was further supported by the detection of these three enzymes in cell membrane fractions in which none of the two aminopeptidases was present.
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页码：285 / 290
页数：6
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