IDENTIFICATION OF MYCOBACTERIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：187

作者：

BUTLER, WR ^{[1
]}

JOST, KC ^{[1
]}

KILBURN, JO ^{[1
]}

机构：

[1] TEXAS DEPT HLTH,BUR LABS,DIV MICROBIOL SERV,AUSTIN,TX 78756

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 11期

关键词：

D O I：

10.1128/JCM.29.11.2468-2472.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.

引用

页码：2468 / 2472

页数：5

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