A LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF FENOPROFEN IN EQUINE PLASMA AND URINE

A high performance liquid chromatographic method to measure plasma and urine fenoprofen levels in equine biofluids is described. Liquid-liquid extraction with diethylether was used to isolate the drug from plasma and urine. The accuracy and reproducibility of the method were within acceptable limits over the concentration range 0-10 mug/mL and 0-20 mug/mL respectively from plasma and urine. Detection limits were 0.05 mug/mL (2 mL plasma) and 0.2 mug/mL (0.5 mL urine). This procedure was applied to ascertain the pharmacokinetics of a 3 g dose of fenoprofen calcium in a horse.