MEASUREMENTS OF MONOVALENT AND DIVALENT COUNTERION DISTRIBUTIONS AROUND PERSISTENCE LENGTH DNA FRAGMENTS IN SOLUTION

被引：33
|
作者：
CHANG, SL
CHEN, SH
RILL, RL
LIN, JS
机构：
[1] MIT,DEPT NUCL ENGN,ROOM 24 211,CAMBRIDGE,MA 02139
[2] MIT,CTR MAT SCI & ENGN,CAMBRIDGE,MA 02139
[3] FLORIDA STATE UNIV,DEPT CHEM,TALLAHASSEE,FL 32306
[4] FLORIDA STATE UNIV,INST MOLEC BIOPHYS,TALLAHASSEE,FL 32306
[5] OAK RIDGE NATL LAB,DIV SOLID STATE,OAK RIDGE,TN 37831
来源：
JOURNAL OF PHYSICAL CHEMISTRY | 1990年 / 94卷 / 21期
关键词：
D O I：
10.1021/j100384a010
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Small-angle X-ray scattering was used to measure the distribution of Tl+ and Ba2+ counterions near the surface of cylindrical, persistence length (500 Å) DNA fragments in aqueous solutions with or without the presence of salts. The absolute scattering intensity distributions were compared with numerical solutions of the nonlinear Poisson-Boltzmann equation in a cell model. Quantitative agreement between calculations and experiments was obtained when we include contributions to the intensity from both the DNA and the diffuse ion cloud. The agreement is slightly better if we assume about 10% of the counterions are within the cylindrical DNA core, namely, in the major and minor grooves of the double helices. © 1990 American Chemical Society.
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页码：8025 / 8028
页数：4
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