ESTABLISHMENT AND CHARACTERIZATION OF 12S ADENOVIRAL E1A IMMORTALIZED RAT SUBMANDIBULAR-GLAND EPITHELIAL-CELLS

被引：17
作者：
HE, XJ
FRANK, DP
TABAK, LA
机构：
[1] UNIV ROCHESTER,SCH MED & DENT,DEPT DENT RES,ROCHESTER,NY 14642
[2] UNIV ROCHESTER,SCH MED & DENT,DEPT BIOCHEM,ROCHESTER,NY 14642
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 01期
关键词：
D O I：
10.1016/0006-291X(90)91279-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rat submandibular gland (RSMG) cells have been immortalized with a retrovirus vector which encodes the adenovirus 12S E1A gene product. The immortalized cells were epithelial-like in nature and displayed a β-adrenergic coupled rise in intracellular cAMP upon exposure to norepinephrine. Western- and lectin-blotting experiments of cell lysates demonstrated the presence of blood group A-reactive oligosaccharides. Such oligosaccharides are characteristic of RSMG mucin-glycoproteins. These cells appear to be suitable for use in analysis of cell-specific factors which regulate RSMG glycosylation. © 1990.
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页码：336 / 343
页数：8
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