BIOSYNTHESIS AND CHARACTERIZATION OF TYPE-X COLLAGEN IN HUMAN FETAL EPIPHYSEAL GROWTH-PLATE CARTILAGE

被引：4

作者：

REGINATO, AM ^{[1
]}

SANZRODRIGUEZ, C ^{[1
]}

JIMENEZ, SA ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT MED,DIV RHEUMATOL,PHILADELPHIA,PA 19107

来源：

OSTEOARTHRITIS AND CARTILAGE | 1995年 / 3卷 / 02期

关键词：

D O I：

10.1016/S1063-4584(05)80043-3

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

We examined in vitro collagen biosynthesis by organ cultures from human fetal epiphyseal growth plate cartilage. The biosynthetic products were characterized by NaCl fractional precipitation, limited proteolytic digestion, and sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. Organ cultures of human fetal epiphyseal growth plate cartilage synthesized large amounts of type X collagen in addition to type II, type IX, and type XI collagens. The individual polypeptide chains of human type X collagen migrated with an apparent M(r) of 45 kDa after proteolytic digestion with pepsin. The migration pattern of these molecules did not change when examined under reducing and nonreducing conditions, indicating that they did not contain intrahelical disulfide bonds. Comparison of the rates at type X collagen biosynthesis at weeks 20 and 24 of human fetal development showed a marked increase of 24 weeks. Northern hybridization analysis of total RNA from freshly isolated epiphyseal growth plate chondrocytes with a cDNA corresponding to the carboxyl terminus of human type X collagen indicated that the developmental increase of type X collagen production is determined by pre-translational mechanisms.

引用

页码：105 / 116

页数：12

共 46 条

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